Furthermore, a preliminary screen of an HIV/HCV antiviral drug mini-library demonstrated the utility of DBF in a HTS assay system. We determined that the fluorescent compound dibenzylfluorescein (DBF) is superior to other compounds in meeting the criteria considered for an efficient HTS assay. Here, we report our testing of various fluorescent probes to assess CYP3A7 activity in a high-throughput manner. Currently, there is no efficient high-throughput screening (HTS) assay to assess CYP3A7 inhibition. Therefore, screening drug candidates for CYP3A7 inhibition is essential to identify chemical entities with potential toxicity risks for neonates. This increases the risk for drug-drug interactions (DDI) and toxicities occurring in the neonate. Despite this, it is generally ignored in the preclinical testing of new drug candidates. In addition to its role metabolizing retinoic acid and the endogenous steroid dehydroepiandrosterone sulfate (DHEA-S), it also has a critical function in drug metabolism and disposition during the first few weeks of life. CYP3A7 is a member of the cytochrome P450 (CYP) 3A enzyme sub-family that is expressed in the fetus and neonate.
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